Dr. Nicolas Tchitchek
Associate Professor in Systems Immunology
Research unit "Immunology-Immunopathology-Immunotherapy" (Inserm UMR-S 959)
Hospital of the Pitié-Salpêtrière — Sorbonne University, Paris, France

Nicolas Tchitchek's Homepage - Research

My research focuses on the design of systems immunology strategies to characterize molecular and cellular interactions in autoimmune and autoinflammatory disorders.

Research activities

I completed my Ph.D. in Systems Biology (Oct. 2008 – Dec. 2011), under the joint supervision of Dr. Annick Lesne and Dr. Arndt Benecke, within the Systems Epigenomics Group at the Institut des Hautes Études Scientifiques in Bures-sur-Yvette Paris. My research focused there on the design of systems biology approaches for the analysis and interpretation of transcriptomic data. Mainly, I applied my research to identify transcriptomic biomarkers associated with the progression of HCV in liver-transplanted patients.

To investigate the transcriptomic and proteomic host responses induced by viral infections, I performed a postdoctoral training in Systems Virology (Feb. 2012 – Mar. 2014) within the laboratory of Prof. Michael Katze at the University of Washington in Seattle. Kinetic aspects are under-considered when characterizing the interplay between viruses and host responses. To overcome these limitations, I devised systems virology strategies for transcriptomic and proteomic analyses centered on host response kinetics. These innovations were developed in the context of: (i) mice infected with respiratory viruses; and (ii) non-human primates infected with viruses causing hemorrhagic fevers. Mainly, I demonstrated the importance of the early molecular host events to control viral infections and to regulate pathogenicity using these animal and viral models. I also pursued my research on the characterization of animal models used in biomedical research, and I worked on the annotation of the Syrian hamster transcriptome.

From April 2014 to April 2019, I was a postdoctoral researcher in Systems Vaccinology within the research unit "Immunology of viral infections and autoimmune diseases" (ImVA unit) lead by Dr. Roger Le Grand at Commissariat à l'Énergie Atomique et aux énergies alternatives of Fontenay-aux-Roses. My project aimed at studying the diversity of cell populations during vaccination and HIV inflammation. In collaboration with Dr. Anne-Sophie Beignon, I applied my research works to predict optimal delays between prime and boost injections for a Modified Vaccinia virus Ankara (MVA) vaccine model on non-human primates. Additionally, I worked with Dr. Elisabeth Menu on metagenomic approaches to explore the diversity of vaginal and rectal microbiotas in cynomolgus macaques.

Since September 2019, I am an Associate Professor in Systems Immunology at Sorbonne University within the research unit "Immunology-Immunopathology-Immunotherapy" (i3 unit) lead by Prof. David Klatzmann at the Hospital of the Pitié-Salpêtrière. I am working to understand the molecular and cellular mechanism involved in autoimmune and autoinflammatory diseases. In details, I am developing approaches to: (i) identify biomarkers associated with diseases severity; (ii) understand diseases at different complexity levels; and (iii) predict patients' responses to treatments. I am also in charge of multiple lectures in the B.Sc. degree "Life Sciences" and in the M.Sc. degree "Systems Immunology" at Sorbonne University.

Since 2014, I am involved within the Franco–Indian laboratory "System Immunology and Genetics of Infectious Diseases" (SIGID) where I am working with Dr. Sylviane Pied to provide breakthroughs in understanding the complexity of host responses to malaria in Indian patients.